Fruit Fly Genetics lab report

 

fruit fly lab report

Genetics of. Drosophila Lab Report Victor Martin April 14, Fourth Block AP Biology INTRODUCTION. Genetics is a topic that has been studied for hundreds of years. One of the most notable geneticists was Gregor Mendel. Gregor Johann Mendels experiments with garden peas dramatically influenced the field of biology. Mendels results became the foundation for the discipline 5/5(9). Genetics Lab Report - Free download as Word Doc .doc /.docx), PDF File .pdf), Text File .txt) or read online for free. Fly Lab. Fly Lab. Search Search. and putting petsronass.gqgaster cultures together. Introduction: The Drosophila melanogaster, the common fruit fly, has been used in genetics since for primary research (Genetics 1/5(1). Report abuse. Mutation in this gene leads to malformation in fruit fly's genitalia. Out in the s, flies were kept in milk bottles in a temporary lab on the outskirts. Cherry fruit fly, feeding responses, Rhagoletis indifferens, sucrose concentrations, survival. Your name will appear on your Lab Report, Quiz, and Certificate of Completion.


Drosophila Virtual Lab


Drosophila melanogaster is a small, common fly found near unripe and rotted fruit. It has been in use for over a century to study genetics and behavior. He was the first to discover sex-linkage and genetic recombination, which placed the small fly in the forefront of genetic research.

Fruit flies are easily obtained from the wild and many biological fruit fly lab report companies carry a variety of different mutations. In addition these companies sell any equipment needed to culture the flies.

Costs are relatively low and most equipment can be used year after year. There are a variety of laboratory exercises one could purchase, although the necessity to do so is questionable. They are small and easily handled. Virgins fruit flies are physically distinctive from mature adults, making it easy to obtain virgin males and females for genetic crosses. Flies have a short generation time days and do well at room temperature. Construct traps to catch wild populations of D.

Gain an understanding of the life cycle of D. Realize many science experiments cannot be conducted and concluded within one or two lab sessions. Hereditary information is located in genes. Combinations of traits can describe the characteristics of fruit fly lab report organism. Communicate and defend a scientific argument.

Therefore, those teachers or students wishing to see where their mutations occur have a ready reference available. Since Drosophila has been so widely used in genetics, there are many different types of mutations available for purchase, fruit fly lab report.

In addition, the attentive student may find mutations within their own wild-caught cultures since, due to a short generation time, mutations are relatively common compared to other animal species. This is the same as the well-known metamorphosis of butterflies.

The larval stage has three instars, or molts. After the eggs hatch, small larvae should be visible in the growing medium. If your media is white, look for the small black area the mouth hooks at the head of the larvae. Some dried premixed media is blue to help identify larvae however this is not a necessity and with a little patience and practice, larvae are easily seen.

In addition, as the larvae feed they disrupt the smooth surface of the media and so by looking only at the surface one can tell if larvae are present. However, it is always a good idea to double check using a stereo microscope. After the third instar, larvae will begin to migrate up the culture vial in order to pupate. Introduction In order to incorporate fruit flies in the classroom, it will be necessary to maintain cultures of flies for manipulation in crosses and as a backup for any mishaps which may occur.

Culturing is very easy and it is recommended to have students maintain their own cultures of flies. In that way, each student or group would be directly responsible for the care and long-term maintenance of the flies, including making large culture populations for their crosses.

When directly involved, students gain proficiency and a greater understanding of the flies requirements and behavior. The teacher should remain as coach, not lecturer, assisting students in techniques. The instructor needs to maintain stock cultures of all strains and mutants used by students in case the aforementioned unforeseeable incident occurs and student cultures die out or become intermixed. Losing cultures is the exception rather than the rule, and as long as students re-culture their flies on a regular basis and no mass contamination occurs, flies can be maintained for decades.

Bottles and vials Thomas Hunt Morgan used glass milk bottles for his experiments and, indeed, any container will do, including baby jars and assorted containers. However, for ease of culturing and transferring cultures, uniform bottles and vials are the best approach.

Both can be purchased from a biological supply store. Bottles are used mainly for the maintenance of large populations of flies whereas culture vials are useful for maintaining smaller populations and are the preferred container for constructing student crosses. If there is a desire to maintain stock cultures for a long period of time, or to reuse bottles and vials it is important completely clean and sterilize them. This is to prevent outbreaks of pests and diseases.

To clean bottle and vials, first freeze them to kill any flies in them. Bottles and vials can be purchased in a variety of sizes and materials.

Glass is effective, however if dropped a student could lose 2 weeks of data in a single spill. Autoclaved sterile plastic vials are available and are fruit fly lab report for student use. Vial sizes range from 96 mm by 25 mm to larger sizes, however the smaller size is recommended for making crosses and maintaining small cultures.

There are a variety of plugs available from soft cotton to foam plugs. This is a matter of preference and costs, however cotton works fine and can be bought at a local drug store in a pinch. Fly food The first step in preparing culture vials is adding food media. There are a variety of types of food available for the flies; some require cooking and others are bought already prepared and dehydrated.

The latter can be purchased from a biological supply company. This is, of course, much quicker and easier than preparing cooked media, fruit fly lab report, so much so that students can fill their own vials with media. However, it must be completely rehydrated for best results, since this is the only water source for adults and larvae.

Therefore, follow the suggestions below to ensure a completely hydrated media:. Add water until media appears completely moistened. Allow the vial to sit for a few minutes, adding additional water if necessary until the media is completely hydrated. The surface should be moist with a shiny appearance and there should be no spaces in the media. If the media is not completed hydrated, production of vigorous cultures is compromised. Flies may be added minutes after media has been hydrated.

Remember to add several grains but not more of yeast to the media surface before adding flies. Keep the media out overnight to cure, keeping the vials covered with cloth to keep fruit fly lab report flies from laying eggs in them. The next day, add yeast and plugs, fruit fly lab report. Refrigerate any unused media vials.

Cooked media can be stored in a refrigerator for several weeks. Allow media to warm to room temperature before adding flies. Do not allow media to dry out. Environment The easiest way to grow flies is at room temperature. In these conditions generation time is shorter days from egg to adult.

Unless equipment is readily available this is unnecessary for successful rearing and crossing of flies. It is preferable to keep flies out of drafts and direct sunlight or heat sources.

These will rapidly fruit fly lab report the media, necessitating fruit fly lab report media changes and the potential to dehydrate the flies. Anesthetizing flies The problem with fruit flies is that they fly!

Therefore a variety of methods have been developed to anesthetize flies. Include are ether, fruit fly lab report, commercial brands such as Flynap, carbon dioxide, and cooling. Each has its strengths and weaknesses. Ether is flammable, has a strong odor and will kill flies if they are over-etherized and can anesthetize younger students!

Flynap, fruit fly lab report, from Carolina Biological, is messy and has an odor that some find offensive. Each of these, however, requires low-cost equipment which can be easily purchased. Carbon dioxide works very well, keeping flies immobile for long periods of time with no side effects, however CO 2 mats blocks are expensive and a CO 2 source usually a bottle and delivery system vials fruit fly lab report clamps are necessary, increasing the costs.

If resourceful, one can use the CO 2 emitted from Alka-Seltzer tablets to anesthetize flies for short periods of time. Set up a large test tube with a tube and stopper system. Add water in the tube, then the Alka-Seltzer tablet. Carbon dioxide gas will be emitted. The least harmful to the flies is either carbon dioxide or cooling anesthetizing. Of these two choices, cooling is the simplest, requiring only a freezer, ice and petri dishes, fruit fly lab report.

In addition, it is the only method which will not affect fly neurology, therefore behavior studies may begin after the flies have warmed up sufficiently.

Anesthetizing flies by cooling In order to incapacitate the flies, place the culture vial in the freezer until the flies are not moving, generally minutes. Dump the flies onto a chilled surface. This can be constructed by using the top of fruit fly lab report petri dish, adding crushed ice, fruit fly lab report, then placing the bottom of the petri dish on top.

Adding flies to this system will keep them chilled long enough to do each experiment, fruit fly lab report. Simply place the flies back into the culture vial when finished. There are no long-lasting side effects to this method, although flies left in the refrigerator too long may not recover.

Another way to keep flies chilled is adding water to zip-lock type freezer bags, place in the freezer with a petri dish nestled on the bag, and allow to freeze. Transferring flies from one vial to another Flies should be transferred every 10 to 14 days.

Students should maintain a backup culture of their flies and the instructor should maintain backup stock cultures of all fly strains. There are two basic ways to transfer flies when forming new cultures. One requires no anesthetizing but quick hands. A Place a funnel in the mouth of a fresh culture vial that already has media added.

In the old vial the one with flies in itgently tap the flies down by softly tamping the vial on a soft fruit fly lab report, such as a mouse pad.

The flies will fall to the bottom and remain there for a few seconds no more than that!

 

An introduction to fruit flies | The Berg Lab

 

fruit fly lab report

 

Genetics of. Drosophila Lab Report Victor Martin April 14, Fourth Block AP Biology INTRODUCTION. Genetics is a topic that has been studied for hundreds of years. One of the most notable geneticists was Gregor Mendel. Gregor Johann Mendels experiments with garden peas dramatically influenced the field of biology. Mendels results became the foundation for the discipline 5/5(9). Genetics Lab Report - Free download as Word Doc .doc /.docx), PDF File .pdf), Text File .txt) or read online for free. Fly Lab. Fly Lab. Search Search. and putting petsronass.gqgaster cultures together. Introduction: The Drosophila melanogaster, the common fruit fly, has been used in genetics since for primary research (Genetics 1/5(1). Lab Report done with Tanuj Sharma Section 1: Biology October 19 th, 2 Drosophila melanogaster is used in this lab as well as many other wet-lab experiments, The eggs of the fruit fly are extremely small, and the transition from embryos within the.